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THE ESCRT COMPLEXES

METHODS AND PROTOCOLS

,
9781493994915 ::  THE ESCRT COMPLEXES
ISBN:

9781493994915

Colección:METHODS IN MOLECULAR BIOLOGY
EditorialSPRINGER NATURE
Edicion:
Idioma:INGLES
P.V.P.: 169,99 € + 4% IVA = 176,79 €
Dto 5% Ahorras 8,84 €
Importe final iva incl. 167,95 €
PLAZO DE ENTREGA 15 DIAS

THIS DETAILED COLLECTION GATHERS BOTH ESTABLISHED AND RECENT TECHNICAL PROCEDURES TO STUDY THE ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT) COMPLEXES IN A WIDE RANGE OF BIOLOGICAL SYSTEMS: ARCHAEA, <I>A. THALIANA</I>, <I>U. MAYDIS</I>, <I>S. CEREVISIAE</I>, <I>S. POMBE</I>, <I>C. ELEGANS</I>, <I>D. MELANOGASTER</I>, AND MAMMALIAN CELLS. OPENING WITH A SECTION ON IMAGING TECHNIQUES, THE BOOK CONTINUES WITH CHAPTERS COVERING BIOCHEMICAL APPROACHES PRESENTING STRATEGIES FOR PRODUCTION AND CHARACTERIZATION OF RECOMBINANT ESCRT PROTEINS, OR OF SPECIFIC ESCRT PROTEIN DOMAINS, AS WELL AS GENETIC AND PROTEOMIC EXPERIMENTAL APPROACHES. WRITTEN FOR THE HIGHLY SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY</I> SERIES, CHAPTERS INCLUDE INTRODUCTION TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.&NBSP;<DIV><BR></DIV><DIV>AUTHORITATIVE AND PRACTICAL, <I>THE ESCRT COMPLEXES: METHODS AND PROTOCOLS</I> SERVES AS A COMPACT GUIDE FOR RESEARCHERS INTERESTED IN ESTABLISHING AN INTEGRATED APPROACH TO INVESTIGATE THE ESCRT MACHINERY FUNCTIONS IN CELL BIOLOGY.</DIV>

<P><B>1. ANALYSIS OF THE ARCHAEAL ESCRT APPARATUS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; RACHEL Y. SAMSON, IAIN G. DUGGIN, AND STEPHEN D. BELL</P><P>&NBSP;</P><P><B>2. GENETIC AND CELL BIOLOGY METHODS TO STUDY ESCRTS IN <I>DROSOPHILA MELANOGASTER</I></B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MARCO GUALTIERI AND THOMAS VACCARI</P><P>&NBSP;</P><P><B>3. FUNCTIONAL ANALYSIS OF ESCRT-POSITIVE EXTRACELLULAR VESICLES IN THE DROSOPHILA WING IMAGINAL DISC</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; TAMAS MATUSEK, PASCAL THÉROND, AND MAXIMILIAN FÜRTHAUER</P><P>&NBSP;</P><P><B>4. SUBCELLULAR LOCALIZATION OF ESCRT-II IN THE NEMATODE C. ELEGANS BY CORRELATIVE-LIGHT ELECTRON MICROSCOPY</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; CÉLINE LARGEAU, EMMANUEL CULETTO, AND RENAUD LEGOUIS</P><P>&NBSP;</P><P><B>5. PROXIMITY LIGATION ASSAY (PLA) TO DETERMINE THE ENDOSOMAL LOCALIZATION OF ESCRT SUBUNIT IN VIRUS-INFECTED CELLS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; BINOD KUMAR, MOHANAN VALIYA VEETTIL, ARUNAVA ROY, AND BALA CHANDRAN</P><P>&NBSP;</P><P><B>6. </B><B>IMMUNO-LOCALIZATION OF ESCRT PROTEINS IN VIRUS-INFECTED CELLS BY FLUORESCENCE AND ELECTRON MICROSCOPY</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; KEISUKE TABATA, ATSUKI NARA, HIROKO OMORI, AND EIJI MORITA</P><P>&NBSP;</P><P><B>7. SINGLE CELL FLUORESCENCE RATIO IMAGE ANALYSIS FOR STUDYING ESCRT FUNCTION IN RECEPTOR TRAFFICKING</B></P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; JALAL M. KAZAN, GERGELY L. LUKACS, PIRJO M. APAJA, AND ARNIM PAUSE</P><P>&NBSP;</P><P><B>8. GENETIC AND BIOCHEMICAL ANALYSES OF YEAST ESCRT</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; SUDEEP BANJADE, SHAOGENG TANG, AND SCOTT D. EMR</P><P>&NBSP;</P><P><B>9. LIVE IMAGING OF ESCRT PROTEINS IN MICROFLUIDICALLY-ISOLATED HIPPOCAMPAL AXONS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; VERONICA BIRDSALL, JOSE C. MARTINEZ, LISA RANDOLPH, ULRICH HENGST, AND CLARISSA L. WAITES</P><P>&NBSP;</P><P><B>10. STUDYING THE SPATIAL ORGANIZATION OF ESCRTS IN CYTOKINETIC ABSCISSION USING THE HIGH RESOLUTION IMAGING TECHNIQUES SIM AND CRYO-SXT</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; SHAI ADAR-LEVOR, INNA GOLIAND, MICHAEL ELBAUM, AND NATALIE ELIA</P>&NBSP;</P><P><B>11. THREE DIMENSIONAL SURFACE RENDERING OF ESCRT PROTEINS MICROSCOPY DATA USING UCSF CHIMERA SOFTWARE</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; ROMAIN LE BARS, MICHELE W. BIANCHI, AND CHRISTOPHE LEFEBVRE</P><P>&NBSP;</P><P><B>12. TRANSIENT EXPRESSION OF ESCRT-COMPONENTS IN ARABIDOPSIS ROOT CELL SUSPENSION CULTURE-DERIVED PROTOPLASTS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MARIE-KRISTIN NAGEL, KARIN VOGEL, AND ERIKA ISONO</P><P>&NBSP;</P><P><B>13. CRYSTALLIZATION AND BIOPHYSICAL APPROACHES FOR STUDYING THE INTERACTIONS BETWEEN THE VPS4-MIT DOMAIN AND ESCRT-III PROTEINS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; TAKAYUKI OBITA, RIEKO KOJIMA, AND MINEYUKI MIZUGUCHI</P><P>&NBSP;</P><P><B>14. BIOCHEMICAL APPROACHES TO STUDYING <I>C. ELEGANS</I> ESCRT FUNCTION IN VITRO</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; SAMUEL BLOCK, AMBER L. SCHUH, AND ANJON AUDHYA</P><P>&NBSP;</P><P><B>15. PURIFICATION OF RECOMBINANT ESCRT-III PROTEINS AND THEIR USE IN ATOMIC FORCE MICROSCOPY AND&NBSP;IN VITRO&NBSP;BINDING AND PHOSPHORYLATION ASSAYS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; LUISA CAPALBO, IOANNA MELA, MARIA ALBA ABAD, A. AROCKIA JEYAPRAKASH, J. MICHAEL EDWARDSON, AND PIER PAOLO D’AVINO</P><P>&NBSP;</P><P><B>16. ASSESSMENT OF ESCRT PROTEIN CHMP5 ACTIVITY ON CLIENT PROTEIN UBIQUITINATION BY IMMUNOPRECIPITATION AND WESTERN BLOTTING</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; FRANCHESKA SON, KATHARINE UMPHRED-WILSON, JAE-HYUCK SHIM, AND STANLEY ADORO</P><P>&NBSP;</P><P><B>17. PURIFICATION OF PLANT ESCRT PROTEINS FOR POLYCLONAL ANTIBODY PRODUCTION</B></P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; JULIO PAEZ-VALENCIA AND MARISA S. OTEGUI</P><P>&NBSP;</P><P><B>18. GENETIC AND CYTOLOGICAL METHODS TO STUDY ESCRT CELL CYCLE FUNCTION IN FISSION YEAST</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; IMANE M. REZIG, SHAUN K. BREMNER, MUSAB S. BHUTTA, IAN P. SALT, GWYN W. GOULD, AND CHRISTOPHER J. MCINERNY</P><P>&NBSP;</P><P><B>19. ESCRT MUTANT ANALYSIS AND IMAGING OF ESCRT COMPONENTS IN THE MODEL FUNGUS <I>USTILAGO MAYDIS</I></B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; CARL HAAG, THOMAS KLEIN, AND MICHAEL FELDBRÜGGE</P><P>&NBSP;</P><P><B>20. GENETIC SUPPRESSOR SCREEN USING AN INDUCIBLE <I>FREE1-RNAI</I> LINE TO DETECT ESCRT GENETIC INTERACTORS IN <I>ARABIDOPSIS</I> </B><B><I>THALIANA</I></B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; QIONG ZHAO, YING ZHU, WENHAN CAO, JINBO SHEN, YONG CUI, SHUXIAN HUANG, AND LIWEN JIANG</P><P>&NBSP;</P><P><B>21. </B><B>SCREENING OF INTERACTIONS WITH THE ESCRT MACHINERY BY A <I>GAUSSIA PRINCEPS</I> SPLIT LUCIFERASE-BASED COMPLEMENTATION ASSAY</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; RINA BAROUCH-BENTOV, YVES JACOB, AND SHIRIT EINAV</P><P>&NBSP;</P><P><B>22. RNA INTERFERENCE-MEDIATED INHIBITION OF ESCRT IN MAMMALIAN CELLS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; KATHERINE BOWERS</P>

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