error
Divisa

POLLEN AND POLLEN TUBE BIOLOGY

METHODS AND PROTOCOLS

9781071606711 ::  POLLEN AND POLLEN TUBE BIOLOGY
ISBN:

9781071606711

Colección:METHODS IN MOLECULAR BIOLOGY
EditorialSPRINGER NATURE
Edicion:
Idioma:INGLES
P.V.P.: 169,99 € + 4% IVA = 176,79 €
Dto 5% Ahorras 8,84 €
Importe final iva incl. 167,95 €
PLAZO DE ENTREGA 15 DIAS

<P>THIS VOLUME EXPLORES A COLLECTION OF EXPERIMENTAL TECHNIQUES USED TO INVESTIGATE DIFFERENT ASPECTS OF POLLEN DEVELOPMENT AND FUNCTION, INCLUDING ITS ROLE IN REACHING THE OVULE AND DELIVERING THE TWO SPERM CELLS. THE TECHNIQUES DISCUSSED RANGE FROM BASIC METHODOLOGY TO CULTIVATE POLLEN IN VITRO TO THE SOPHISTICATED EXPERIMENTS INVOLVING MICROMANIPULATION, LAB-ON-CHIP TECHNOLOGY, OR HIGH-END IMAGING. THE CHAPTERS IN THIS BOOK COVER TOPICS SUCH AS POLLEN GRAIN COUNTING USING A CELL COUNTER; RESTRICTED POLLINATION FOR TRACING INDIVIDUAL POLLEN TUBES IN A PISTIL; OBTAINING MUTANT POLLEN FOR PHENOTYPIC ANALYSIS AND POLLEN TUBE DUAL-STAINING; ANALYZING INTRACELLULAR GRADIENTS IN POLLEN TUBES; AND MEASURING EXOCYTOSIS RATE IN ARABIDOPSIS POLLEN TUBE USING CORRECTED FLUORESCENCE RECOVERY AFTER PHOTOCONVERSION (CFRAPC) TECHNIQUE. WRITTEN IN THE HIGHLY SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY</I> SERIES FORMAT, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.<BR><BR>CUTTING-EDGE AND THOROUGH, <I>POLLEN AND POLLEN TUBE BIOLOGY: METHODS AND PROTOCOLS</I> IS A VALUABLE RESOURCE FOR NOVICE AND EXPERT SCIENTISTS INTERESTED IN LEARNING MORE ABOUT THE FIELD OF PLANT REPRODUCTION.&NBSP;</P><P><BR></P>

<P>PREFACE…<BR>TABLE OF CONTENTS…<BR>CONTRIBUTING AUTHORS…<BR><BR>1. POLLEN GRAIN COUNTING USING A CELL COUNTER<BR>HIROYUKI KAKUI, MISAKO YAMAZAKI, NAOTO-BENJAMIN HAMAYA, AND KENTARO K. SHIMIZU<BR><BR>2. A TOOLKIT FOR TEASING APART THE EARLY STAGES OF POLLEN-STIGMA INTERACTIONS IN ARABIDOPSIS THALIANA<BR>HYUN KYUNG LEE, STUART MACGREGOR, AND DAPHNE R. GORING<BR><BR>3. DECAPITATION CROSSES TO TEST POLLEN FERTILITY MUTATIONS FOR DEFECTS IN STIGMA-STYLE PENETRATION<BR>CHRYSTLE WEIGAND AND JEFFREY HARPER<BR><BR>4. ISOLATION OF THE PISTIL-STIMULATED POLLEN TUBE SECRETOME<BR>SAID HAFIDH AND DAVID HONYS<BR><BR>5. RESTRICTED POLLINATION FOR TRACING INDIVIDUAL POLLEN TUBES IN A PISTIL<BR>TARO TAKAHASHI, TOSHIYUKI MORI, AND TOMOKO IGAWA<BR><BR>6. SEMI-IN VIVO ASSAY FOR POLLEN TUBE ATTRACTION<BR>SHENG ZHONG, ZHIJUAN WANG, AND LI-JIA QU<BR><BR>7. ISOLATION AND CLONING OF SUPPRESSOR MUTANTS WITH IMPROVED POLLEN FERTILITY<BR>STEVEN BEUDER AND CORA A. MACALISTER<BR><BR>8. WORKFLOW TO CHARACTERIZE MUTANTS WITH REPRODUCTIVE DEFECTS<BR>JENNIFER A. NOBLE AND RAVISHANKAR PALANIVELU<BR><BR>9. INTERNALLY-CONTROLLED METHODS TO QUANTIFY POLLEN TUBE GROWTH AND PENETRATION DEFECTS IN ARABIDOPSIS THALIANA<BR>DEVIN K. SMITH AND IAN S. WALLACE<BR><BR>10. ARABIDOPSIS THALIANA POLLEN TUBE CULTURE FOR MULTI-OMICS STUDIES<BR>M. COSTA, J. SILVA, AND S. COIMBRA<BR><BR>11. LARGE SCALE ANALYSIS OF POLLEN VIABILITY AND OXIDATIVE LEVEL USING H2DCFDA-STAINING COUPLED WITH FLOW CYTOMETRY<BR>NICHOLAS RUTLEY AND GAD MILLER<BR><BR>12. OBTAINING MUTANT POLLEN FOR PHENOTYPIC ANALYSIS AND POLLEN TUBE DUAL-STAINING<BR>SHENG ZHONG, ZHIJUAN WANG, AND LI-JIA QU<BR><BR>13. GALVANOTROPIC CHAMBER FOR CONTROLLED REORIENTATION OF POLLEN TUBE GROWTH AND SIMULTANEOUS CONFOCAL IMAGING OF INTRACELLULAR DYNAMICS<BR>FIRAS BOU DAHER AND ANJA GEITMANN<BR><BR>14. ANALYZING INTRACELLULAR GRADIENTS IN POLLEN TUBES<BR>DANIEL S. C. DAMINELI, MARIA TERESA PORTES, AND JOSÉ A. FEIJÓ<BR><BR>15. SILICONE CHAMBERS FOR POLLEN TUBE IMAGING IN MICROSTRUCTURES IN VITRO ENVIRONMENTS<BR>HANA BERTRAND-RAKUSOVÁ, YOUSSEF CHEBLI, AND ANJA GEITMANN<BR><BR>16. ANALYZING THE IMPACT OF PROTEIN OVEREXPRESSION ON CA2+ DYNAMICS AND DEVELOPMENT IN TOBACCO POLLEN TUBES<BR>CHUNXIA ZHANG, LEONIE STEINHORST, AND JÖRG KUDLA<BR><BR>17. IMAGING AND ANALYSIS OF THE CONTENT OF CALLOSE, PECTIN, AND CELLULOSE IN THE CELL WALL OF ARABIDOPSIS POLLEN TUBES GROWN IN VITRO<BR>ANA R. SEDE, DIEGO L. WENGIER, CECILIA BORASSI, JOSÉ M. ESTEVEZ, AND JORGE P. MUSCHIETTI<BR><BR>18. CHARACTERIZATION OF GROWTH BEHAVIOR AND THE RESULTING FORCES APPLIED BY POLLEN TUBES IN A 3D MATRIX<BR>RONNY REIMANN AND DELF KAH<BR><BR>19. FLOW CHAMBER ASSAY TO IMAGE THE RESPONSE OF FRET-BASED NANOSENSORS IN POLLEN TUBES TO CHANGES IN MEDIUM COMPOSITION<BR>THERESA MARIA REIMANN<BR><BR>20. QUANTIFICATION OF MECHANICAL FORCES AND PHYSIOLOGICAL PROCESSES INVOLVED IN POLLEN TUBE GROWTH USING MICROFLUIDICS AND MICROROBOTICS<BR>JAN BURRI, GAUTAM MUNGLANI, BRADLEY J. NELSON, UELI GROSSNIKLAUS, AND HANNES VOGLER<BR><BR>21. MEASURING EXOCYTOSIS RATE IN ARABIDOPSIS POLLEN TUBE USING CORRECTED FLUORESCENCE RECOVERY AFTER PHOTOCONVERSION (CFRAPC) TECHNIQUE<BR>JINGZHE GUO AND ZHENBIAO YANG<BR><BR>22. TESTING POLLEN TUBE PROTEINS FOR IN VIVO BINDING TO PHOSPHATIDIC ACID BY N0BUTANOL TREATMENT AND CONFOCAL MICROSCOPY<BR>CAROLIN FRITZ AND BENEDIKT KOST<BR><BR>SUBJECT INDEX LIST…</P><DIV><BR></DIV>

BOTANICA
Libros relacionados
ISBN: 9781071601785 PLANT EPIGENETICS AND EPIGENOMICSPLANT EPIGENETICS AND EPIGENOMICS
9781071601785
Febrero 2020

159.99€ S/I
151,99€ S/I
ISBN: 9780128126288 PLANT SYSTEMATICSPLANT SYSTEMATICS
9780128126288
Enero 2020

91.95€ S/I
87,35€ S/I
ISBN: 9781444392159 PLANT TRANSFORMATION TECHNOLOGIESPLANT TRANSFORMATION TECHNOLOGIES
9781444392159
Julio 2020

Oferta estudiantes

146.54€ S/I
139,21€ S/I

ISBN: 9783030289393 ETHNOBOTANY OF THE MOUNTAIN REGIONS OF FAR EASTERN EUROPEETHNOBOTANY OF THE MOUNTAIN REGIONS OF ...
9783030289393
Julio 2020

399.99€ S/I
379,99€ S/I

ISBN: 9783030449285 MEDICINAL AND AROMATIC PLANTS OF NORTH AMERICAMEDICINAL AND AROMATIC PLANTS OF NORTH ...
9783030449285
Julio 2020

129.99€ S/I
123,49€ S/I