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PLURIPOTENT STEM-CELL DERIVED CARDIOMYOCYTES

9781071614839 ::  PLURIPOTENT STEM-CELL DERIVED CARDIOMYOCYTES
ISBN:

9781071614839

Colección:METHODS IN MOLECULAR BIOLOGY
Volumen:2320
EditorialSPRINGER NATURE
Edicion:
Idioma:INGLES
P.V.P.: 159,99 € + 4% IVA = 166,39 €
Dto 5% Ahorras 8,32 €
Importe final iva incl. 158,07 €
PLAZO DE ENTREGA 15 DIAS

<P>THIS VOLUME PROVIDES METHODOLOGIES FOR ES AND IPS CELL TECHNOLOGY ON THE STUDY OF CARDIOVASCULAR DISEASES. CHAPTERS GUIDE READERS THROUGH PROTOCOLS ON CARDIOMYOCYTE GENERATION FROM PLURIPOTENT STEM CELLS, PHYSIOLOGICAL MEASUREMENTS, BIOINFORMATIC ANALYSIS, GENE EDITING TECHNOLOGY, AND CELL TRANSPLANTATION STUDIES. WRITTEN IN THE HIGHLY SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY </I>SERIES FORMAT, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.</P><P>&NBSP;</P><P>AUTHORITATIVE AND CUTTING-EDGE, PLURIPOTENT STEM-CELL DERIVED CARDIOMYOCYTES AIMS TO HELP RESEARCHERS SET UP EXPERIMENTS USING PLURIPOTENT STEM CELL-DERIVED CARDIAC CELLS.</P><BR>

<P>PART I: OVERVIEW</P><P>1. MAKING CARDIOMYOCYTES FROM PLURIPOTENT STEM CELLS&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>PETER KARAGINIUS AND YOSHINORI YOSHIDA</P><P>&NBSP;</P>PART II: GENERATION OF PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES AND CARDIAC TISSUES<P></P><P>2. A METHOD FOR LARGE-SCALE CARDIAC DIFFERENTIATION, PURIFICATION, AND CARDIAC SPHEROID PRODUCTION OF HUMAN INDUCED PLURIPOTENT STEM CELLS&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>YUIKA MORITA, SHUGO TOHYAMA, JUN FUJITA, AND KEIICHI FUKUDA</P>&NBSP;<P></P><P>3. LARGE-SCALE DIFFERENTIATION OF STEM CELL-DERIVED CARDIOMYOCYTES BY STIRRING-TYPE SUSPENSION CULTURE&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>NAGAKO SOUGAWA, SHIGERU MIYAGAWA, AND YOSHIKI SAWA</P><P>&NBSP;</P>4. EFFICIENT METHOD TO DISSOCIATE INDUCED PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTE AGGREGATES INTO SINGLE CELLS&NBSP;&NBSP;&NBSP; <P></P><P>EMIKO ITO, SHIGERU MIYAGAWA, YOSHINORI YOSHIDA, AND YOSHIKI SAWA</P><P>&NBSP;</P><P>5. ISOLATION OF CARDIOMYOCYTES DERIVED FROM HUMAN PLURIPOTENT STEM CELLS USING MIRNA SWITCHES&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>KENJI MIKI, HIROHIDE SAITO, AND YOSHINORI YOSHIDA</P><P>&NBSP;</P><P>6. FABRICATION OF CARDIAC CONSTRUCTS USING BIO-3D PRINTER&NBSP;&NBSP;&NBSP; </P><P>KENICHI ARAI, DAIKI MURATA, SHOKO TAKAO, AND KOICHI NAKAYAMA</P><P>&NBSP;</P><P>7. FABRICATION OF THICK AND ANISOTROPIC CARDIAC TISSUE ON NANOFIBROUS SUBSTRATE FOR REPAIRING INFARCTED MYOCARDIUM&NBSP;&NBSP;&NBSP; </P><P>JUNJUN LI, LI LIU, ITSUNARI MINAMI, SHIGERU MIYAGAWA, AND YOSHIKI SAWA</P><P>&NBSP;</P>8. CONSTRUCTION OF THREE-DIMENSIONAL CARDIAC TISSUES USING LAYER BY LAYER METHOD&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; <P></P><P>MAKI TAKEDA, SHIGERU MIYAGAWA, MITSURU AKASHI, AND YOSHIKI SAWA</P><P>&NBSP;</P><P>9. GENERATION OF CYLINDRICAL ENGINEERED CARDIAC TISSUES FROM HUMAN IPS CELL-DERIVED CARDIOVASCULAR CELL LINEAGES&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>HIDETOSHI MASUMOTO</P><P>&NBSP;</P><P>PART III: PHYSIOLOGICAL MESUREMENTS USING PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES</P><P>&NBSP;</P>10. PROTOCOL FOR MORPHOLOGICAL AND FUNCTIONAL PHENOTYPE ANALYSIS OF HIPS-DERIVED CARDIOMYOCYTES&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; <P></P><P>JUN LI AND JONG-KOOK LEE</P><P>&NBSP;</P><P>11. APPLICATION OF FLUOVOLT MEMBRANE POTENTIAL DYE FOR INDUCED PLURIPOTENT STEM CELL-DERIVED CARDIAC SINGLE CELLS AND MONOLAYERS DIFFERENTIATED VIA EMBRYOID BODIES&NBSP; </P><P>TADASHI TAKAKI AND YOSHINORI YOSHIDA</P><P>&NBSP;</P><P>12. MULTI-ELECTRODE ARRAY ASSAYS USING HUMAN INDUCED PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>DAISUKE YOSHINAGA, YIMIN WURIYANGHAI, AND TAKERU MAKIYAMA</P><P>&NBSP;</P><P>13. ELECTROPHYSIOLOGICAL ANALYSIS OF HIPSC-DERIVED CARDIOMYOCYTES USING A PATCH-CLAMP TECHNIQUE&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>YUTA YAMAMOTO, SAYAKO HIROSE, YIMIN WURIYANGHAI, DAISUKE YOSHINAGA, AND TAKERU MAKIYAMA</P><P>&NBSP;</P><P>14. CHARACTERIZATION OF VENTRICULAR AND ATRIAL CARDIOMYOCYTE SUBTYPES FROM HUMAN INDUCED PLURIPOTENT STEM CELLS&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>MISATO KOAKUTSU, TADASHI TAKAKI, KENJI MIKI, AND YOSHINORI YOSHIDA</P><P>&NBSP;</P><P>15. ASSESSMENT OF CONTRACTILITY IN HUMAN IPS CELL-DERIVED CARDIOMYOCYTES USING MOTION VECTOR ANALYSIS&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>YASUNARI KANDA, AYANO SATSUKA, SAYO HAYASHI, MIHOKO HAGIWARA-NAGASAWA, AND ATSUSHI SUGIYAMA</P><P>&NBSP;</P><P>16. CONTRACTILE FORCE MEASUREMENT OF ENGINEERED CARDIAC TISSUES DERIVED FROM HUMAN IPS CELLS</P>DAISUKE SASAKI, KATSUHISA MATSUURA, AND TATSUYA SHIMIZU<P></P><P>&NBSP;</P><P>17. A METHOD FOR CONTRACTION FORCE MEASUREMENT OF IPSC DERIVED ENGINEERED CARDIAC TISSUES</P><P>YUYA FUJIWARA, KOHEI DEGUCHI, KENJI MIKI, TOMOYUKI NISHIMOTO, AND YOSHINORI YOSHIDA </P>&NBSP;<P></P><P>PART IV: TRANSCRIPTOME AND BIOINFOMATICS ANALYSIS</P><P>18. SINGLE-CARDIOMYOCYTE RNA SEQUENCING TO DISSECT THE MOLECULAR PATHOPHYSIOLOGY OF THE HEART</P><P>MANAMI KOTOH, SEITARO NOMURA, SHINTARO YAMADA,HIROYUKI ABURATANI, AND ISSEI KOMURO</P>&NBSP;<P></P><P>19. RNA-SEQUENCING ANALYSIS OF DIFFERENTIALLY EXPRESSED GENES IN HUMAN IPSC-DERIVED CARDIOMYOCYTES&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>CHIKAKO OKUBO, MEGUMI NARITA, TAKUYA YAMAMOTO, AND YOSHINORI YOSHIDA</P><P>&NBSP;</P>20. ANALYSIS OF TRANSCRIPTIONAL PROFILING OF CHAMBER-SPECIFIC HUMAN CARDIAC MYOCYTES DERIVED FROM PLURIPOTENT STEM CELLS<P></P><P>ANTONIO LUCENA-CACACE AND YOSHINORI YOSHIDA</P><P>&NBSP;</P><P>PART V: GENE EDITING AND CRISPR TECHNOLOGY FOR PLURIPOTENT STEM CELLS</P>21. GENOME EDITING IN HUMAN INDUCED PLURIPOTENT STEM CELLS (HIPSCS) &NBSP;&NBSP; <P></P><P>SHUICHIRO HIGO, SHUNGO HIKOSO, SHIGERU MIYAGAWA, AND YASUSHI SAKATA</P><P>&NBSP;</P><P>22. GENERATION OF EFFICIENT KNOCK-IN MOUSE AND HUMAN PLURIPOTENT STEM CELLS USING CRISPR-CAS9&NBSP;&NBSP;&NBSP;&NBSP; </P><P>TATSUYA ANZAI, HIROMASA HARA, CHANTHRA NAWIN, TAKETARO SADAHIRO, MASAKI IEDA, YUTAKA HANAZONO, AND HIDEKI UOSAKI</P><P>&NBSP;</P><P>23. CRISPRI/A SCREENING WITH HUMAN IPSCS&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>MASATAKA NISHIGA, LEI S. QI, AND JOSEPH C. WU</P><P>&NBSP;</P><P>PART VI: TRANSPLANTATION OF PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES</P><P>24. TRANSPLANTATION OF HUMAN INDUCED PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES IN A MOUSE MYOCARDIAL INFARCTION MODEL&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; </P><P>TAKESHI HATANI AND YOSHINORI YOSHIDA</P><P>&NBSP;</P><P>25. TRANSPLANTATION OF PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES INTO A MYOCARDIAL INFARCTION MODEL OF CYNOMOLGUS MONKEY&NBSP; </P><P>HIDEKI KOBAYASHI, HAJIME ICHIMURA, NOBUROU OHASHI, AND YUJI SHIBA</P>

ANALISIS CLINICOS
BIOLOGIA
BIOLOGIA CELULAR
BIOQUIMICA / BIOLOGIA MOLECULAR
GENETICA
MEDICINA INTERNA
MEDICINA PREVENTIVA
TERMINOLOGIA MEDICA
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