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MATRIX METALLOPROTEASES

METHODS AND PROTOCOLS

9781493968619 ::  MATRIX METALLOPROTEASES
ISBN:

9781493968619

Colección:METHODS IN MOLECULAR BIOLOGY
EditorialSPRINGER NATURE
Edicion:
Páginas:346
Idioma:INGLES
Oferta estudiantes
P.V.P.: 149,99 € + 4% IVA = 155,99 €
Dto 5% Ahorras 7,80 €
Importe final iva incl. 148,19 €
PLAZO DE ENTREGA 15 DIAS

<P>SINCE THE IDENTIFICATION OF THE FIRST MATRIX METALLOPROTEASE (INSTERSTITIAL COLLAGENASE OR MMP-1) MORE THAN 20 CLOSELY RELATED AND EVOLUTIONARILY CONSERVED VERTEBRATE MMPS HAVE BEEN DISCOVERED. CHAPTERS IN THIS VOLUME DETAIL METHODS AND PROTOCOLS ON RECENT ADVANCES IN THE EXPRESSION AND PURIFICATION OF MMPS, BIOPHYSICAL METHODS SUCH AS X-RAY CRYSTALLOGRAPHY, NMR SPECTROSCOPY AND SMALL ANGLE X-RAY SCATTERING, COMPUTATIONAL AND EXPERIMENTAL METHODS FOR DETERMINING MMP SPECIFICITY, METHODS FOR DETECTING MMP ACTIVITY <I>IN VITRO</I> AND <I>IN VIVO</I>, AND METHODS FOR THE DEVELOPMENT AND CHARACTERIZATION OF MMP-BASED INHIBITORS AS POTENTIAL THERAPEUTICS FOR THE TREATMENT OF VARIOUS DISEASES. WRITTEN IN THE HIGHLY SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY </I>SERIES FORMAT, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.</P><BR> AUTHORITATIVE AND PRACTICAL, <I>MATRIX METALLOPROTEASES: METHODS AND PROTOCOLS </I>AIMS TO ENSURE SUCCESSFUL RESULTS IN THE FURTHER STUDY OF THIS VITAL FIELD.

<P><B>PART I EXPRESSION AND PURIFICATION OF MATRIX METALLOPROTEASES</B></P><P>&NBSP;1. EXPRESSION AND PURIFICATION OF MATRIX METALLOPROTEINASES IN <I>ESCHERICHIA COLI</I></P><P>KRISHNA K. SINGH, RUCHI JAIN, HARINI RAMANAN, AND DEEPAK K. SAINI</P><P>&NBSP;</P><P>2. EXPRESSION AND PURIFICATION OF A MATRIX METALLOPROTEASE TRANSMEMBRANE DOMAIN IN <I>ESCHERICHIA COLI</I></P><P>CHARLES A. GALEA</P><P>&NBSP;</P><P>3. HETEROLOGOUS EXPRESSION OF THE ASTACIN PROTEASE MEPRIN B IN <I>PICHIA PASTORIS</I></P><P>DAGMAR SCHLENZIG AND STEPHAN SCHILLING</P><P>&NBSP;</P><P>&NBSP;</P><P><B>PART II STRUCTURAL CHARACTERIZATION OF MATRIX METALLOPROTEASES</B></P><P>&NBSP;4. STRUCTURAL STUDIES OF MATRIX METALLOPROTEINASE BY X-RAY DIFFRACTION</P><P>ELENA DECANETO, WOLFGANG LUBITZ, AND HIDEAKI OGATA</P><P>&NBSP;</P><P>5. MAPPING LIPID BILAYER RECOGNITION SITES OF METALLOPROTEINASES AND OTHER PROSPECTIVE PERIPHERAL MEMBRANE PROTEINS</P><P>TARA C. MARCINK, RAMA K. KOPPISETTI, YAN G. FULCHER, AND STEVEN R. VAN DOREN</P><P>&NBSP;</P><P>6. USING SMALL ANGLE X-RAY SCATTERING (SAXS) TO CHARACTERISE THE SOLUTION CONFORMATION AND FLEXIBILITY OF MATRIX METALLOPROTEINASES (MMPS)</P>LOUISE E. BUTT, ROBERT A. HOLLAND, NIKUL S. KHUNTI, DEBRA L. QUINN, AND ANDREW R. PICKFORD<P></P><P>&NBSP;</P><P>&NBSP;</P><P><B>PART III COMPUTATIONAL SIMULATIONS OF MATRIX METALLOPROTEASES</B></P><P>&NBSP;7. MOLECULAR DYNAMICS STUDIES OF MATRIX METALLOPROTEASES</P><P>NATALIA DÍAZ AND DIMAS SUÁREZ</P><P>&NBSP;</P><P>&NBSP;</P><P><B>PART IV DETERMINING MATRIX METALLOPROTEASE SUBSTRATE SPECIFICITY</B></P><P>&NBSP;8. DETERMINING THE SUBSTRATE SPECIFICITY OF MATRIX METALLOPROTEASES USING FLUOROGENIC PEPTIDE SUBSTRATES</P><P>MACIEJ J. STAWIKOWSKI, ANNA M. KNAPINSKA, AND GREGG B. FIELDS</P><P>&NBSP;</P><P>9. TIME-RESOLVED ANALYSIS OF MATRIX METALLOPROTEINASE SUBSTRATES IN COMPLEX SAMPLES</P>PASCAL SCHLAGE, FABIAN E. EGLI, AND ULRICH AUF DEM KELLER<P></P><P>&NBSP;<P>10. IDENTIFICATION OF PROTEASE CLEAVAGE SITES BY CHARGE-BASED ENRICHMENT OF PROTEIN N-TERMINI</P><P>ZON W. LAI AND OLIVER SCHILLING</P><P>&NBSP;</P><P>11. MAPPING THE SUBSTRATE RECOGNITION LANDSCAPES OF METALLOPROTEASES USING COMPREHENSIVE MUTAGENESIS</P><P>COLIN A. KRETZ</P><P>&NBSP;</P><P>&NBSP;</P><P><B>PART V DETECTION OF MATRIX METALLOPROTEASES</B></P><P>&NBSP;12. DETECTION OF MATRIX METALLOPROTEINASES BY ZYMOGRAPHY</P><P>RAJEEV B. TAJHYA, RUTVIK S. PATEL, AND CHRISTINE BEETON</P><P>&NBSP;</P><P>13. IMAGING MATRIX METALLOPROTEASES IN SPONTANEOUS COLON TUMORS:&NBSP; VALIDATION BY CORRELATION WITH HISTOPATHOLOGY&LT;</P><P>HARVEY HENSLEY, HARRY S. COOPER, WEN-CHI L. CHANG, AND MARGIE L. CLAPPER</P><P>&NBSP;</P>&NBSP; PART VI MATRIX METALLOPROTEASE INHIBITORS &NBSP;14. VIRTUAL HIGH-THROUGHPUT SCREENING FOR MATRIX METALLOPROTEINASE INHIBITORS JUN YONG CHOI, AND RITA FUERST<P></P>&NBSP; 15. COMPUTATIONAL APPROACHES TO MATRIX METALLOPROTEASE DRUG DESIGN <P>TANYA SINGH, B. JAYARAM, AND OLAYIWOLA ADEDOTUN ADEKOYA<SUP></SUP></P><P>&NBSP;</P><P>16. A SIMPLE, ADAPTABLE BLOOD-BRAIN BARRIER CELL MODEL FOR SCREENING MATRIX METALLOPROTEINASE INHIBITOR FUNCTIONALITY</P><P>JENNIFER S. MYERS, JOAN HARE, AND QING-XIANG AMY SANG</P><P>&NBSP;</P>&NBSP;<P></P><P><B>PART VII MATRIX METALLOPROTEASES AS </B><B>BIOMARKERS</B><B><SUP></SUP></B></P><P>&NBSP;17. MATRIX METALLOPROTEASES AS BIOMARKERS OF DISEASE</P><P>FERNANDO LUIZ AFFONSO FONSECA, BEATRIZ DA COSTA AGUIAR ALVES, LIGIA AJAIME AZZALIS, AND THAÍS MOURA GÁSCON BELARDO<SUP></SUP></P><P>&NBSP;</P>

BIOQUIMICA / BIOLOGIA MOLECULAR
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