ANALYTICAL TECHNIQUES IN BIOCHEMISTRY

<P></P><P>THIS BOOK PROVIDES A COMPREHENSIVE OVERVIEW OF THE MAJOR BIOCHEMICAL ANALYTICAL TECHNIQUES, WITH DETAILED DESCRIPTIONS OF THE INSTRUMENTATION AND APPLICATIONS. THE CONTRIBUTIONS, WHICH EACH FOCUS ON A SPECIFIC TECHNIQUE, ARE BASED ON A THOROUGH REVIEW AND ANALYSIS OF THE CURRENT LITERATURE AS WELL AS THE AUTHORS’ EXPERIENCES IN THE LAB.&NBSP;</P><P>DIVIDED INTO NINE PARTS, THE BOOK PROVIDES INSIGHTS INTO BASIC SEPARATION TECHNIQUES LIKE SEDIMENTATION, FILTRATION AND CENTRIFUGATION, AS WELL AS ANALYTICAL TECHNIQUES SUCH AS SPECTROPHOTOMETRY, CHROMATOGRAPHY, ELECTROPHORESIS, IMMUNO-TECHNIQUES, RADIOACTIVITY AND MICROSCOPY.&NBSP;</P><P></P>

<DIV>&NBSP;1 SEDIMENTATION</DIV><DIV> 1.1 GRAVITY SEDIMENTATION</DIV><DIV> 1.2 ZONE SETTLING &COMPRESSION</DIV><DIV>2 FILTRATION&NBSP; &NBSP; &NBSP; &NBSP; &NBSP; &NBSP; &NBSP;</DIV><DIV> 2.1 MICROFILTRATION</DIV><DIV> 2.2 ULTRAFILTRATION</DIV><DIV>3 CENTRIFUGATION </DIV><DIV> 3.1 TWO BASIC MODES OF CENTRIFUGATION</DIV><DIV> 3.2 INSTRUMENT DESIGN</DIV><DIV>4 COLORIMETRY AND SPECTROPHOTOMETER (SPECTROPHOTOMETRY)</DIV><DIV> 4.1 COLORIMETRY</DIV><DIV> 4.2 SPECTROPHOTOMETRY</DIV><DIV>5 CHROMATOGRAPHY</DIV><DIV> 5.1 PARTITION CHROMATOGRAPHY</DIV><DIV> &NBSP; &NBSP; &NBSP; &NBSP; &NBSP;5.1.1&NBSP;&NBSP;PAPER CHROMATOGRAPHY</DIV><DIV> &NBSP; &NBSP; &NBSP; &NBSP; &NBSP;5.1.2 THIN LAYER CHROMATOGRAPHY</DIV><DIV> &NBSP; &NBSP; &NBSP; &NBSP; &NBSP;5.1.3&NBSP;HPLC</DIV><DIV>&NBSP; &NBSP; &NBSP; &NBSP; &NBSP; &NBSP; &NBSP; &NBSP; &NBSP;5.1.4&NBSP;&NBSP;GAS CHROMATOGRAPHY</DIV><DIV> &NBSP; &NBSP; &NBSP; &NBSP; &NBSP;5.1.5 GEL FILTRATION CHROMATOGRAPHY</DIV><DIV>6 ELECTROPHORESIS</DIV><DIV><BR></DIV><DIV> 6.1 PAPER ELECTROPHORESIS</DIV><DIV><BR></DIV><DIV> 6.2 SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS</DIV><DIV><BR></DIV><DIV> 6.3 IMMUNOELECTROPHORESIS</DIV><DIV><BR></DIV><DIV>7 IMMUNO-TECHNIQUES</DIV><DIV><BR></DIV><DIV> 7.1 IMMUNODIFFUSION</DIV><DIV><BR></DIV><DIV> 7.2 IMMUNO-FLUORESCENCE&NBSP;</DIV><DIV><BR></DIV><DIV> 7.3 IMMUNOASSAY: ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA)&NBSP;</DIV><DIV><BR></DIV><DIV> 7.4 RADIOIMMUNOASSAY (RIA)</DIV><DIV><BR></DIV><DIV> 7.5 WESTERN BLOTTING OR IMMUNOBLOTTING&NBSP;</DIV><DIV><BR></DIV><DIV> 7.6 SOUTHERN BLOTTING</DIV><DIV><BR></DIV><DIV> 7.7 NORTHERN BLOT</DIV><DIV><BR></DIV><DIV>8. RADIOACTIVITY</DIV><DIV><BR></DIV><DIV>9. MICROSCOPY</DIV><DIV><BR></DIV>