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T-CELL RECEPTOR SIGNALING

METHODS AND PROTOCOLS

9781071602652 ::  T-CELL RECEPTOR SIGNALING
ISBN:

9781071602652

Collection:METHODS IN MOLECULAR BIOLOGY
Publisher:SPRINGER NATURE
Edition:
Pages:296
Language:INGLES
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<P>THIS VOLUME PROVIDES CURRENT AND NEW ADVANCED METHODS AND PROTOCOLS TO STUDY T CELLS. CHAPTERS GUIDE READERS THROUGH T CELL DIVERSITY USING MASS CYTOMETRY, ANALYZING T CELLS FROM SINGLE CELL LEVEL, CRISPR/CAS9 TECHNIQUES TO STUDY THE T CELL ACTIVATION, TECHNIQUES TO STUDY SUBSETS OF TCELL’S, PROCEDURES TO STUDY ARTIFICIAL ANTIGEN PRESENTOSOMES FOR T CELL ACTIVATION, TECHNIQUES TO STUDY THE T CELL DEVELOPMENT, TWO-PHOTON MICROSCOPY, AND MAIT CELLS. WRITTEN IN THE HIGHLY SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY </I>SERIES FORMAT, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.</P><P>&NBSP;AUTHORITATIVE AND CUTTING-EDGE, <I>T-CELL RECEPTOR SIGNALING: METHODS AND PROTOCOLS </I>AIMS TO PROVIDE A WIDE RANGE OF APPROACHES AND BE AN INVALUABLE RESOURCE FOR PRESENT AND FUTURE GENERATIONS OF T CELL RESEARCHERS.</P><P></P>

<P>1. EXPLORATION OF T CELL DIVERSITY USING MASS CYTOMETRY</P><P>KAITLIN C O’BOYLE, TAKUYA OHTANI, BERTRAM BENGSCH, SASIKANTH MANNE, SARAH E HENRICKSON, E JOHN WHERRY,<SUP> </SUP>AND CECILE ALANIO</P><P>&NBSP;</P><P>2. ANALYSIS OF TRANSCRIPTIONAL PROFILING OF IMMUNE CELLS AT THE SINGLE-CELL LEVEL</P><P>ANNABEL FERGUSON AND KONG CHEN</P><P>&NBSP;</P><P>3. SIMULTANEOUS MEASUREMENT OF SURFACE PROTEINS AND GENE EXPRESSION FROM SINGLE CELLS</P><P>JIADI LUO, CARLA A. ERB, AND KONG CHEN</P><P>&NBSP;</P><P>4. A CARRIER STRATEGY FOR MASS CYTOMETRY ANALYSIS OF SMALL NUMBERS OF CELLS</P><P>XIAN JIA, XIAOJUAN ZHOU, HAIPING ZHENG, SHAN JIANG, JIANNAN WENG, LEI HUANG, ZHIQIANG DU, CHANGCHUN XIAO, LEI ZHANG, XIAO LEI CHEN, AND GUO FU<SUP></SUP></P><P>&NBSP;</P><P>5. CRISPR/CAS9-BASED GENETIC SCREENING TO STUDY T CELL FUNCTION</P>WANJING SHANG, FEI WANG, QI ZHU<SUP> </SUP>, LIANGYU WANG, AND HAOPENG WANG<SUP> </SUP><P></P><P>&NBSP;</P><P>6. PREFERENTIAL EXPANSION OF CD4<SUP>+</SUP>FOXP3<SUP>+</SUP> REGULATORY T CELLS (TREGS) IN VITRO BY TUMOR NECROSIS FACTOR</P><P>CHON-KIT CHOU AND XIN CHEN<SUP></SUP></P><P>&NBSP;</P><P>7. CD4<SUP>+</SUP> T CELL DIFFERENTIATION <I>IN VITRO</I> </P><P>WENYONG YANG, XUEYING CHEN, AND HONGBO HU</P><P>&NBSP;</P><P>8. CHARACTERIZATION OF IMMUNE CELL SUBSET EXPANSION IN RESPONSE TO THERAPEUTIC TREATMENT IN MICE</P><P>JAKUB TOMALA AND JAMIE B. SPANGLER<SUP></SUP></P><P>&NBSP;</P><P>9 PRIMARY T CELL TRANSDUCTION TO STUDY FOLLICULAR HELPER T CELL DIFFERENTIATION</P><P>YANG ZHANG, XUEHUI LONG, AND XIAOMING WANG</P><P>&NBSP;</P>10. <I>IN VITRO</I> GENERATION OF STEM CELL MEMORY-LIKE T CELLS FROM ACTIVATED T CELLS<P></P><P>MAKOTO ANDO, MARI IKEDA AND AKIHIKO YOSHIMURA, AND TAISUKE KONDO</P><P>&NBSP;</P><P>11. ARTIFICIAL ANTIGEN PRESENTOSOMES FOR T CELL ACTIVATION</P><P>YI-GENG PANG, CHIEN-CHUNG CHANG</P><P>&NBSP;</P><P>12. IMAGING CHIMERIC ANTIGEN RECEPTOR (CAR) -TRIGGERED T CELL ACTIVATION</P><P>KENDRA LIBBY AND XIAOLEI SU</P><P>&NBSP;</P><P>13. ASSESSING THE IMPACT OF PHYTOCHEMICALS ON IMMUNE CHECKPOINTS: IMPLICATIONS FOR CANCER IMMUNOTHERAPY</P><P>MELANIE R. POWER COOMBS AND DAVID W. HOSKIN</P><P>14. TESTING THE EFFICIENCY AND KINETICS OF NEGATIVE SELECTION USING THYMIC SLICES</P><P>TYNG-AN ZHOU, CHIA-LIN HSU, AND IVAN LILYANOV DZHAGALOV</P><P>&NBSP;</P><P>15. INVESTIGATING T CELL RECEPTOR SIGNALING DYNAMICS <I>IN SITU</I> BY TWO-PHOTON MICROSCOPY OF THYMOCYTES EXPRESSING GENETIC REPORTERS IN LOW-DENSITY CHIMERAS</P><P>MARILAINE FOURNIER, MENGQI DONG, AND HEATHER J. MELICHAR</P><P>&NBSP;</P><P>16. RETROVIRAL GENE TRANSDUCTION INTO T-CELL PROGENITORS FOR ANALYSIS OF T CELL DEVELOPMENT IN THE THYMUS</P><P>RYUNOSUKE MURO, HIROSHI TAKAYANAGI, AND TAKESHI NITTA</P><P>&NBSP;</P>17. AN INTEGRATED STRATEGY FOR IDENTIFYING TARGETS OF UBIQUITIN-MEDIATED DEGRADATION IN CD4+ T CELLS<P></P><P>NATANIA S. FIELD, CLAIRE E. O’LEARY, JOSEPH M. DYBAS, HUA DING, AND PAULA M. OLIVER</P><P>&NBSP;</P><P>18. RADIOISOTOPE BASED PROTOCOL FOR DETERMINATION OF CENTRAL CARBON METABOLISM IN T CELLS</P><P>XUYONG CHEN, JOHN SHERMAN, AND RUONING WANG</P><P>&NBSP;</P><P>19. ASSESSMENT OF IMMUNE PROTECTIVE T CELL REPERTOIRE IN HUMANS IMMUNIZED WITH NOVEL TUBERCULOSIS VACCINES</P><P>MANGALAKUMARI JEYANANTHAN AND ZHOU XING<SUP></SUP></P><P><SUP>&NBSP;</SUP></P><P>20. STUDYING PERIPHERAL T CELL HOMEOSTASIS IN MICE; A CONCISE TECHNICAL REVIEW</P><P>MOUTUAATA M. MOUTUOU, SIMON-DAVID GAUTHIER, NICOLAS CHEN, DOMINIQUE LEBOEUF, AND MARTIN GUIMOND </P><P>&NBSP;</P><P>21. DETECTION, EXPANSION, AND ISOLATION OF HUMAN MAIT CELLS</P><P>YU LIU, WEI WANG, XIONGWEN WU AND XIUFANG WENG </P><P>&NBSP;</P><P>22. <I>IN VITRO</I> DIFFERENTIATION OF CD4<SUP>+</SUP> T CELL EFFECTOR AND REGULATORY SUBSETS</P><P>JACLYN R. ESPINOSA, JOSHUA D. WHEATON, AND MARIA CIOFANI</P><P></P><P></P>

BIOCHEMISTRY / MOLECULAR BIOLOGY
IMMUNOLOGY
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