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SYNTHETIC ANTIBODIES

METHODS AND PROTOCOLS

9781493968558 ::  SYNTHETIC ANTIBODIES
ISBN:

9781493968558

Collection:METHODS IN MOLECULAR BIOLOGY
Publisher:SPRINGER NATURE
Edition:
Pages:460
Language:INGLES
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THIS DETAILED VOLUME PRESENTS A SET OF PROTOCOLS USEFUL FOR RESEARCHERS IN THE FIELD OF RECOMBINANT IMMUNOGLOBULIN AND ALTERNATIVE SCAFFOLD ENGINEERING, APTAMER DEVELOPMENT, AND GENERATION OF MOLECULARLY IMPRINTED POLYMERS (MIPS). PART I INCLUDES METHODS THAT DEAL WITH AMINO-ACID BASED SYNTHETIC ANTIBODIES. BRIEF PROTOCOLS ABOUT THE GENERATION OF ANTIBODY LIBRARIES ARE DETAILED, AS WELL AS TECHNIQUES FOR ANTIBODY SELECTION, CHARACTERIZATION, AND VALIDATION. THIS SECTION IS COMPLETED BY A BRIEF DESCRIPTION OF A BIOINFORMATICS PLATFORM THAT SUPPORTS ANTIBODY ENGINEERING DURING RESEARCH AND DEVELOPMENT. PART II CONTAINS BASIC PROCEDURES ABOUT THE SELECTION AND CHARACTERIZATION OF APTAMER MOLECULES, AND PART III DESCRIBES FUNDAMENTAL PROCESSES OF MIP GENERATION AND APPLICATION. WRITTEN FOR THE HIGHLY SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY</I> SERIES, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.&NBSP;<DIV><BR></DIV><DIV>AUTHORITATIVE AND PRACTICAL, <I>SYNTHETIC ANTIBODIES: METHODS AND PROTOCOLS</I> IS AN IDEAL GUIDE FOR SCIENTISTS SEEKING TO PROPEL THE VITAL STUDY OF ANTIBODY RESEARCH.<P></P><P></P><P></P></DIV>

<P><B>PART I: AMINO ACID-BASED SYNTHETIC ANTIBODIES</B></P><P><B>&NBSP;</B></P><P><B>1. ANTIBODY MIMETICS, PEPTIDES, AND PEPTIDOMIMETICS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; XIAOYING ZHANG AND THIRUMALAI DIRAVIYAM</P><P>&NBSP;</P><P><B>2. CONSTRUCTION OF A SCFV LIBRARY WITH SYNTHETIC, NON-COMBINATORIAL CDR DIVERSITY</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; XUELIAN BAI AND HYUNBO SHIM</P><P>&NBSP;</P><P><B>3. ENZYMATIC ASSEMBLY FOR SCFV LIBRARY CONSTRUCTION</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MIEKO KATO AND YOSHIRO HANYU</P><P>&NBSP;</P><B>4. DIRECTED EVOLUTION OF PROTEIN THERMAL STABILITY USING YEAST SURFACE DISPLAY</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MICHAEL W. TRAXLMAYR AND ERIC V. SHUSTA</P><P>&NBSP;</P><P><B>5. WHOLE CELL PANNING WITH PHAGE DISPLAY</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; YVONNE STARK, SOPHIE VENET, AND ANNIKA SCHMID</P><P>&NBSP;</P><P><B>6. GENERATING CONFORMATION AND COMPLEX-SPECIFIC SYNTHETIC ANTIBODIES</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MARCIN PADUCH AND ANTHONY A. KOSSIAKOFF</P><P>&NBSP;</P><P><B>7. HIGH-THROUGHPUT IGG CONVERSION OF PHAGE DISPLAYED FAB ANTIBODY FRAGMENTS BY AMPLYFAST</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; ANDREA STERNER AND CAROLIN ZEHETMEIER</P><P>&NBSP;</P><P><B>8. UTILIZATION OF SELENOCYSTEINE FOR SITE-SPECIFIC ANTIBODY CONJUGATION</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; XIULING LI AND CHRISTOPH RADER</P><P>&NBSP;</P><P><B>9. SOLUBILITY CHARACTERIZATION AND IMAGING OF INTRABODIES USING GFP-FUSIONS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; EMILIE RENAUD, PIERRE MARTINEAU, AND LAURENCE GUGLIELMI</P><P>&NBSP;</P><P><B>10. </B><B>ANTIBODY VALIDATION BY IMMUNOPRECIPITATION FOLLOWED BY MASS SPECTROMETRY ANALYSIS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; HELENA PERSSON, CHARLOTTA PREGER, EDYTA MARCON, JOHAN LENGQVIST, AND SUSANNE GRÄSLUND</P><P>&NBSP;</P><P><B>11. NOVEL HPLC-BASED SCREENING METHOD TO ASSESS DEVELOPABILITY OF ANTIBODY-LIKE MOLECULES</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; NEERAJ KOHLI AND MELISSA L. GEDDIE</P><P>&NBSP;</P><P><B>12. GLYCOSYLATION PROFILING OF </B><B>?/</B><B>? T CELL RECEPTOR CONSTANT DOMAINS EXPRESSED IN MAMMALIAN CELLS</B></P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; KAI ZHANG, STEPHEN J. DEMAREST, XIUFENG WU, AND JONATHAN R. FITCHETT</P><P>&NBSP;</P><P><B>13. A PROXIMITY-BASED ASSAY FOR IDENTIFICATION OF LIGAND AND MEMBRANE PROTEIN INTERACTION IN LIVING CELLS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; HONGKAI ZHANG AND RICHARD A. LERNER</P><P>&NBSP;</P><P><B>14. A BIOTIN LIGASE-BASED ASSAY FOR THE QUANTIFICATION OF THE CYTOSOLIC DELIVERY OF THERAPEUTIC PROTEINS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; WOUTER P.R. VERDURMEN, MARIGONA MAZLAMI, AND ANDREAS PLÜCKTHUN</P><P>&NBSP;</P><P><B>15. DATA-DRIVEN ANTIBODY ENGINEERING USING GENEDATA BIOLOGICS™</B></P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MARIA WENDT AND GUIDO CAPPUCCILLI</P><P>&NBSP;</P><P><B>PART II: NUCLEOTIDE-BASED SYNTHETIC ANTIBODIES: APTAMERS</B></P><P><B>&NBSP;</B></P><P><B>16. SELECTION OF APTAMERS AGAINST WHOLE LIVING CELLS: FROM CELL-SELEX TO IDENTIFICATION OF BIOMARKERS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; NAM NGUYEN QUANG, ANNA MIODEK, AGNES CIBIEL, AND FRÉDÉRIC DUCONGÉ</P><P>&NBSP;</P><P><B>17. RAPID SELECTION OF RNA APTAMERS THAT ACTIVATE FLUORESCENCE OF SMALL MOLECULES</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; GRIGORY S. FILONOV</P><P>&NBSP;</P><P><B>18. AN ENZYME-LINKED APTAMER SORBENT ASSAY TO EVALUATE APTAMER BINDING</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MATTHEW D. MOORE, BLANCA I. ESCUDERO, AND LEE-ANN JAYKUS</P><P>&NBSP;</P><P><B>19. INCORPORATING APTAMERS IN THE MULTIPLE ANALYTE PROFILING ASSAYS (XMAP): DETECTION OF C-REACTIVE PROTEIN</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; ELYSE D. BERNARD, KATHY C. NGUYEN, MARIA C. DEROSA, AZAM F. TAYABALI, AND ROCIO ARANDA-RODRIGUEZ</P><P>&NBSP;</P><P><B>PART III: MOLECULARY IMPRINTED POLYMERS</B></P><P><B>&NBSP;</B></P><P><B>20. TRANSFERRING THE SELECTIVITY OF A NATURAL ANTIBODY INTO A MOLECULARLY IMPRINTED POLYMER</B></P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; ROMANA SCHIRHAGL</P><P>&NBSP;</P><P><B>21. </B><B>PREPARATION OF MOLECULARLY IMPRINTED MICROSPHERES BY PRECIPITATION POLYMERIZATION</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; TIBOR RENKECZ AND VIOLA HORVATH</P><P>&NBSP;</P><P><B>22. GENERATION OF JANUS MOLECULARLY IMPRINTED POLYMER PARTICLES</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; XIANTAO SHEN AND CHUIXIU HUANG</P><P>&NBSP;</P><P><B>23. SURFACE ENGINEERING OF NANOPARTICLES TO CREATE SYNTHETIC ANTIBODIES</B></P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; LINDA CHIO, DARWIN YANG, AND MARKITA LANDRY</P><P>&NBSP;</P><P><B>24. H5N1 VIRUS PLASTIC ANTIBODY BASED ON MOLECULARLY IMPRINTED POLYMERS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; CHAK SANGMA, PETER A. LIEBERZEIT, AND WANNISA SUKJEE</P><P>&NBSP;</P><P><B>25. REPLACEMENT OF ANTIBODIES IN PSEUDO-ELISAS: MOLECULARLY IMPRINTED NANOPARTICLES FOR VANCOMYCIN DETECTION</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; FRANCESCO CANFAROTTA, KATARZYNA SMOLINSKA-KEMPISTY, AND SERGEY PILETSKY</P><P>&NBSP;</P><P><B>26. CELL AND TISSUE IMAGING WITH MOLECULARLY IMPRINTED POLYMERS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MARIA PANAGIOTOPOULOU, STEPHANIE KUNATH, KARSTEN HAUPT, AND BERNADETTE TSE SUM BUI</P>

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