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NEURAL CREST CELLS

METHODS AND PROTOCOLS

,
9781493994113 ::  NEURAL CREST CELLS
ISBN:

9781493994113

Col·lecció:METHODS IN MOLECULAR BIOLOGY
EditorialSPRINGER NATURE
Edició:
Idioma:INGLES
P.V.P.: 159,99 € + 4% IVA = 166,39 €
Dto 5% Estalvies 8,32 €
Import final iva incl. 158,07 €
Entrega en 15 dies

<P></P><P>THIS DETAILED BOOK PROVIDES A WIDE RANGE OF TECHNIQUES, FROM THOSE THAT HAVE BEEN USED EXTENSIVELY SINCE THE VERY FIRST INVESTIGATIONS INTO NEURAL CREST CELLS TO THOSE THAT ARE CURRENTLY CUTTING-EDGE, IN ORDER TO EXPLORE THE DEVELOPMENT OF NEURAL CREST CELLS IN HUMAN, MICE, RAT, CHICK, QUAIL, MEDAKA, AND SHARK. WITH A BIT OF IMAGINATION AND ADJUSTMENT, MANY OF THESE METHODOLOGIES CAN BE ADAPTABLE TO ANY SPECIES DESIRABLE FOR STUDY. WRITTEN FOR THE HIGHLY SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY</I> SERIES FORMAT, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.</P><P><BR></P>AUTHORITATIVE AND PRACTICAL, <I>NEURAL CREST CELLS: METHODS AND PROTOCOLS</I> SERVES AS AN IDEAL REFERENCE GUIDE FOR ASPIRING AND EXPERIENCED DEVELOPMENTAL BIOLOGISTS ALIKE.<P></P><P></P>

<P>1.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; THE NEURAL CREST: A REMARKABLE MODEL SYSTEM FOR STUDYING DEVELOPMENT AND DISEASE (CHAYA KALCHEIM) </P><P>2.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; ADDRESSING INTERDISCIPLINARY DIFFICULTIES IN DEVELOPMENTAL BIOLOGY / MATHEMATICAL COLLABORATIONS: A NEURAL CREST EXAMPLE (DONALD NEWGREEN, KERRY LANDMAN AND JAMES OSBORNE)</P><P>3.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; GENERATION OF NEURAL CREST PROGENITORS FROM HUMAN PLURIPOTENT STEM CELLS (KWAKU DAD ABU-BONSRAH, SERENA VIVENTI, DONALD NEWGREEN AND MIRELLA DOTTORI)</P>4.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; FULLY DEFINED AND XENO-FREE INDUCTION OF HPSCS INTO NEURAL CREST USING TOP-DOWN INHIBITION OF BMP SIGNALING (JAMES HACKLAND, TOM FRITH AND PETER ANDREWS)<P></P><P>5.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; DECIPHERING THE NEURAL CREST CONTRIBUTION TO CEPHALIC DEVELOPMENT WITH AVIAN EMBRYOS (MOUSSAB ALRAJEH, ZUZANA VAVRUSOVA AND SOPHIE CREUZET)</P><P>6.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; IN OVO ELECTROPORATION OF PLASMID DNA AND MORPHOLINOS INTO SPECIFIC TISSUES DURING EARLY EMBRYOGENESIS (REBECCA MCLENNAN AND PAUL KULESA)</P><P>7.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; EX VIVO CULTURE AND MANIPULATION OF MOUSE NEURAL CREST CELLS FROM PRIMARY EMBRYONIC TISSUE EXPLANTS (SOPHIE WISZNIAK)</P><P>8.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; TECHNOLOGIES FOR LIVE IMAGING OF ENTERIC NEURAL CREST DERIVED CELLS (MARLENE HAO, ANNETTE BERGNER, DONALD F. NEWGREEN, HIDEKI ENOMOTO AND HEATHER YOUNG)</P><P>9.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MOUSE EMBRYO CULTURE FOR THE STUDY OF NEURAL CREST CELLS (WILLIAM MUNOZ AND PAUL TRAINOR)</P><P>10.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; LASER CAPTURE MICRODISSECTION OF MURINE EMBRYONIC NEURAL CREST CELLS (ROBERT GREENE, IRINA SMOLENKOVA AND MICHELE PISANO)</P><P>11.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; IN VIVO AND IN VITRO QUANTITATIVE ANALYSIS OF NEURAL CREST CELL MIGRATION (ELIAS BARRIGA, ADAM SHELLARD AND ROBERTO MAYOR)</P><P>12.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; PROTEOMICS IN NEURAL CREST CELL RESEARCH (PETER MCCARTHY AND QUENTEN SCHWARZ)</P><P>13.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; NEURAL CREST METHODOLOGIES IN ZEBRAFISH AND MEDAKA (KLEIO PETRATOU, KAREN CAMARGO-SOSA, RUQAIYA AL JABRI, YUSUKE NAGAO AND ROBERT KELSH)</P><P>14.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; FLUORESCENCE-ACTIVATED CELL SORTING AND NANOSTRING PROFILING OF SINGLE NEURAL CREST CELLS AND PIGMENT CELLS (TATIANA SUBKHANKULOVA AND ROBERT KELSH)</P><P>15.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; LIVE IMAGING OF NEURAL CREST AND PIGMENT CELLS AND TRANSIENT TRANSGENIC MANIPULATION OF GENE ACTIVITY (REYNA DEEYA BALLIM, YUSUKE NAGAO AND ROBERT KELSH)</P><P>16.&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; AVENUES FOR INVESTIGATING THE NEURAL CREST AND ITS DERIVATIVES IN NON-MODEL (UNCONVENTIONAL) VERTEBRATES: A CRANIOFACIAL SKELETON PERSPECTIVE (MICHEAL DEPEW AND FEDERICA BERTOCCHINI)</P>

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