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MOLECULAR TOXICOLOGY PROTOCOLS

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9781071602225 ::  MOLECULAR TOXICOLOGY PROTOCOLS
ISBN:

9781071602225

Col·lecció:METHODS IN MOLECULAR BIOLOGY
EditorialSPRINGER NATURE
Edició:
Pàgines:576
Idioma:INGLES
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THIS FULLY REVISED BOOK INCLUDES NEW AND IMPROVED PROTOCOLS TO ANALYZE TOXICITY AT THE DNA, RNA, AND PROTEIN LEVELS USING GENOMIC, EPIGENOMIC, TRANSCRIPTOMIC, AND PROTEOMIC APPROACHES. SECTIONS OF THE BOOK ADDRESS KEY SUBJECTS SUCH AS BIOINFORMATICS AND BIOSTATISTICS, TOXICANT-INDUCED LUNG INFLAMMATION AND TUMORS, ANALYSIS OF PROTEIN AND GENE EXPRESSION AND SILENCING, GENE PROMOTER METHYLATION, DETECTION OF CHROMOSOMAL AND GENOME-WIDE DAMAGE, ANALYSIS OF DNA-ADDUCTS AND SURROGATE GENE MUTATIONS, DETECTION AND CHARACTERIZATION OF CANCER GENE MUTATION, AND MANY OTHER AREAS OF STUDY. WRITTEN FOR THE HIGHLY SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY</I> SERIES, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.&NBSP;<DIV><BR></DIV><DIV>COMPREHENSIVE AND UP-TO-DATE, <I>MOLECULAR TOXICOLOGY PROTOCOLS, THIRD EDITION</I> WILL PROVE TO PLAY AN IMPORTANT ROLE IN GENERATING A BETTER UNDERSTANDING OF THE FIELD OF TOXICOLOGY AND ITS SUB-DISCIPLINES.</DIV>

<P><B>PART I: BIOINFORMATICS AND BIOSTATISTICS</B></P><P><B>&NBSP;</B></P><P><B>1. BIOENERGETIC ANALYSES OF IN VITRO AND IN VIVO SAMPLES TO GUIDE TOXICOLOGICAL ENDPOINTS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; JONATHAN W. BOYD, JULIA A. PENATZER, NICOLE PRINCE, JULIE V. MILLER, ALICE A. HAN, AND HOLLY N. CURRIE</P><P>&NBSP;</P><P><B>2. NEXT GENERATION SEQUENCING (NGS) APPLICATION IN MULTI-PARAMETER GENE EXPRESSION ANALYSIS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; DEGENG WANG AND ANDREY L. KARAMYCHEV</P><P>&NBSP;</P><P><B>3. INTEGRATED CHIP-SEQ AND RNA-SEQ DATA ANALYSIS COUPLED WITH BIOINFORMATICS APPROACHES TO INVESTIGATE REGULATORY LANDSCAPE OF TRANSCRIPTION MODULATORS IN BREAST CANCER CELLS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; JAIRO RAMOS, QUENTIN FELTY, AND DEODUTTA ROY</P>&NBSP;<P></P><P><B>4. ANALYSIS OF RNA SEQUENCING DATA USING CLC GENOMICS WORKBENCH</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; CHIA-HSIN LIU AND Y. PETER DI</P><P>&NBSP;</P><P><B>PART II: TOXICANT-INDUCED LUNG INFLAMMATION AND LUNG TUMORS</B></P><P><B>&NBSP;</B></P><P><B>5. USING BRONCHOALVEOLAR LAVAGE TO EVALUATE CHANGES IN PULMONARY DISEASES</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MARISSA E. DI, DANDAN YANG, AND Y. PETER DI</P><P>&NBSP;</P><P><B>6. FAST AND EFFICIENT MEASUREMENT OF CLINICAL AND BIOLOGICAL SAMPLES USING IMMUNOASSAY-BASED MULTIPLEXING SYSTEMS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; YINGZE ZHANG, XIAOYUN LI, AND Y. PETER DI</P><P>&NBSP;</P><P><B>7. INDUCTION OF LUNG TUMORS AND MUTATIONAL ANALYSIS IN FVB/N MICE TREATED WITH THE TOBACCO CARCINOGEN 4-(METHYLNITROSAMINO)-1-(3-PYRIDYL)-1-BUTANONE</B></P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; NATALIE J. ROTHENBERGER AND LAURA P. STABILE<P></P><P>&NBSP;</P><P><B>PART III: ANALYSIS OF PROTEIN AND GENE EXPRESSION AND SILENCING</B></P><P><B>&NBSP;</B></P><P><B>8. TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS: A GEL-BASED PROTEOMIC APPROACH FOR PROTEIN ANALYSIS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; WEIMIN GAO</P><P>&NBSP;</P><P><B>9. DETERMINATION AND QUANTIFICATION OF BACTERIAL VIRULENT GENE EXPRESSION USING QUANTITATIVE REAL TIME PCR</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; QIAO LIN AND Y. PETER DI</P><P>&NBSP;</P><P><B>10. USING A LENTIVIRUS-BASED INDUCIBLE RNAI VECTOR TO SILENCE A GENE</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; SI CHEN, DONGYING LI, ZHEN REN, DIANKE YU, BAITANG NING, NAN MEI, AND LEI GUO</P><P>&NBSP;</P><P><B>PART IV: GENE PROMOTER METHYLATION</B></P><P>&NBSP;</P><P><B>11. ANALYSIS OF TOXICANTS-INDUCED ALTERATIONS IN DNA METHYLATION BY METHYLATION-SENSITIVE RANDOM AMPLIFIED POLYMORPHIC DNA-POLYMERASE CHAIN REACTION (MS-RAPD-PCR)</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; KAMALESHWAR P. SINGH</P><P>&NBSP;</P><P><B>12. DETECTION OF DNA METHYLATION BY MEDIP AND MBDCAP ASSAYS: AN OVERVIEW OF TECHNIQUES</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; HANG-KAI HSU, YU-I WENG, PEI-YIN HSU, TIM H.-M. HUANG, AND YI-WEN HUANG</P><P>&NBSP;</P><P><B>PART V: DETECTION OF CHROMOSOMAL AND GENOME-WIDE DAMAGE</B></P><P><B>&NBSP;</B></P><P><B>13. ANALYSIS OF TELOMERE LENGTH AND ABERRATIONS BY QUANTITATIVE FISH</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; ELISE FOUQUEREL AND PATRICIA OPRESKO</P><P>&NBSP;</P><P><B>14. DETECTION OF LOSS OF HETEROZYGOSITY IN <I>TK-</I>DEFICIENT MUTANTS FROM L5178Y <I>TK</I><SUP>+/–</SUP>-3.7.2C MOUSE LYMPHOMA CELLS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; XIAOQING GUO, YING CHEN, MARTHA M. MOORE, AND NAN MEI</P><P>&NBSP;</P><P><B>15. DETECTION OF DNA DOUBLE-STRAND BREAKS AND CHROMOSOME TRANSLOCATIONS USING LIGATION-MEDIATED PCR AND INVERSE PCR</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; SHEETAL SINGH, SHYH-JEN SHIH, AND ANDREW T. M. VAUGHAN</P>&NBSP;<P></P><P><B>PART VI: ANALYSIS OF DNA-ADDUCTS AND SURROGATE GENE MUTATIONS</B></P><P><B>&NBSP;</B></P><P><B>16. <SUP>32</SUP>P-POSTLABELING ANALYSIS OF DNA ADDUCTS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; DAVID H. PHILLIPS AND VOLKER M. ARLT</P><P>&NBSP;</P><P><B>17. DETERMINATION OF MUTATIONAL SPECTRA INDUCED BY ENVIRONMENTAL TOXICANTS IN COMPLEX HUMAN CELL POPULATIONS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; ROBERT GEALY AND PHOUTHONE KEOHAVONG</P><P>&NBSP;</P><P><B>18. DETECTION OF <I>PIG-A</I> MUTANT ERYTHROCYTES IN THE PERIPHERAL BLOOD OF RATS AND MICE</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; VASILY N. DOBROVOLSKY, XUEFEI CAO, JAVED A. BHALLI, AND ROBERT H. HEFLICH</P><P>&NBSP;</P><P><B>19. ANALYSIS OF IN VIVO MUTATION IN THE <I>HPRT</I> AND <I>TK</I> GENES OF MOUSE LYMPHOCYTES</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; VASILY N. DOBROVOLSKY, JOSEPH G. SHADDOCK, AND ROBERT H. HEFLICH</P><P>&NBSP;</P><P><B>20. MOLECULAR ANALYSIS OF MUTATIONS IN THE HUMAN <I>HPRT</I> GENE</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; PHOUTHONE KEOHAVONG, LIQIANG XI, AND STEPHEN G. GRANT</P><P>&NBSP;</P><P><B>21. SIMULTANEOUS QUANTIFICATION OF T(14;18) AND <I>HPRT</I> EXON 2/3 DELETIONS IN HUMAN LYMPHOCYTES</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; JAMES C. FUSCOE</P><P>&NBSP;</P><P><B>PART VII: DETECTION AND CHARACTERIZATION OF CANCER GENE MUTATION</B></P><P><B>&NBSP;</B></P><P><B>22. ANALYSIS OF MUTATIONS IN K-RAS AND P53 GENES IN SPUTUM AND PLASMA SAMPLES</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; LIFANG ZHANG, WEI-MIN GAO, AND PHOUTHONE KEOHAVONG</P>&NBSP;<P></P><P><B>23. ACB-PCR QUANTIFICATION OF LOW-FREQUENCY HOTSPOT CANCER-DRIVER MUTATIONS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MEAGAN B. MYERS, KAREN L. MCKIM, YIYING WANG, MALATHI BANDA, AND BARBARA L. PARSONS</P><P>&NBSP;</P><P><B>24. DETECTION AND CHARACTERIZATION OF ONCOGENE MUTATIONS IN PRENEOPLASTIC AND EARLY NEOPLASTIC LESIONS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; TOSHINARI MINAMOTO</P><P>&NBSP;</P><P><B>PART VIII: ANALYSIS OF DNA DAMAGE AND REPAIR MECHANISMS</B></P><P><B>&NBSP;</B></P><P><B>25. THE SISTER CHROMATID EXCHANGE (SCE) ASSAY</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; DAWN M. STULTS, MICHAEL W. KILLEN, PAOLA MARCO-CASANOVA, AND ANDREW J. PIERCE</P><P>&NBSP;</P><P><B>26. THE GENE CLUSTER INSTABILITY (GCI) ASSAY FOR RECOMBINATION</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MICHAEL W. KILLEN, DAWN M. STULTS, PAOLA MARCO-CASANOVA, AND ANDREW J. PIERCE</P><P>&NBSP;</P><P><B>27. NEW PERSPECTIVES ON UNSCHEDULED DNA SYNTHESIS:&NBSP; FUNCTIONAL ASSAY FOR GLOBAL GENOMIC DNA NUCLEOTIDE EXCISION REPAIR</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; MANASI R. PIMPLEY, MEGAN L. FOLEY, AND JEAN J. LATIMER</P><P>&NBSP;</P><P><B>28. HOST CELL REACTIVATION: ASSAY FOR ACTIVELY TRANSCRIBED DNA NUCLEOTIDE EXCISION REPAIR USING LUCIFERASE FAMILY EXPRESSION VECTORS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; JOWAHER S. ALANAZI AND JEAN J. LATIMER</P><P>&NBSP;</P><P><B>PART IX: ANALYSIS OF ENDOPLASMIC RETICULUM STRESS AND MIRNA/RNA INTERACTION</B></P><P><B>&NBSP;</B></P><P><B>29. METHODS FOR ESTABLISHING AND USING A STABLE CELL LINE EXPRESSING BOTH GAUSSIA LUCIFERASE AND FIREFLY LUCIFERASE TO SCREEN FOR ENDOPLASMIC RETICULUM STRESS</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; ZHEN REN, SI CHEN, AND LEI GUO</P><P>&NBSP;</P><P><B>30. FREMSA: A METHOD THAT PROVIDES DIRECT EVIDENCE OF THE INTERACTION BETWEEN MICRORNA AND MRNA</B></P><P>&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP;&NBSP; DIANKE YU, SI CHEN, DONGYING LI, BRIDGETT KNOX, LEI GUO, AND BAITANG NING</P>

BIOLOGIA
BIOQUIMICA / BIOLOGIA MOLECULAR
FARMACOLOGIA
GENETICA
TOXICOLOGIA
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