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GROUP A STREPTOCOCCUS

METHODS AND PROTOCOLS

,
9781071604663 ::  GROUP A STREPTOCOCCUS
ISBN:

9781071604663

Col·lecció:METHODS IN MOLECULAR BIOLOGY
EditorialSPRINGER NATURE
Edició:
Pàgines:402
Idioma:INGLES
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Dto 5% Estalvies 8,84 €
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PART I: GENOTYPING AND GENETIC MANIPULATION OF GROUP A STREPTOCOCCUS1. DETECTION OF STREPTOCOCCUS PYOGENES VIRULENCE FACTORSALEKSANDRA KOZINSKA AND IZABELA SITKIEWICZ 2. CLOSED-TUBE MULTIPLEX REAL-TIME PCR FOR THE DETECTION OF GROUP A STREPTOCOCCAL SUPERANTIGENSNOUR ZAHI GAMMOH AND LOTHAR RINK 3. THE EMM-CLUSTER TYPING SYSTEMPIERRE R. SMEESTERS AND ANNE BOTTEAUX 4. PROTOCOLS FOR TN-SEQ ANALYSES IN THE GROUP A STREPTOCOCCUSYOANN LE BRETON, ASHTON T. BELEW, AND KEVIN S. MCIVER 5. GENETIC MANIPULATION OF GROUP A STREPTOCOCCUS - GENE DELETION BY ALLELIC REPLACEMENTTIMOTHY C. BARNETT, JESSICA N. DAW, MARK J. WALKER, AND STEPHAN BROUWER 6. GENERATION OF BIOLUMINESCENT GROUP A STREPTOCOCCUS FOR BIOPHOTONIC IMAGINGJACELYN M. S. LOH, KAR YAN SOH, AND THOMAS PROFT PART II: APPLICATION OF GROUP A STREPTOCOCCUS OMICS7. WHOLE GENOME SEQUENCE ANALYSIS AND POPULATION GENOMICS OF GROUP A STREPTOCOCCI JAKE A LACEY, TAYLAH B JAMES, STEVEN YC TONG, AND MARK R DAVIES 8. GROUP A STREPTOCOCCUS TRANSCRIPTOME ANALYSISMISU SANSON AND ANTHONY R. FLORES 9. PROTOCOL FOR PROTEOME ANALYSIS OF GROUP A STREPTOCOCCUSLAURA WILK 10. INVESTIGATION OF GROUP A STREPTOCOCCAL INTERACTIONS WITH HOST GLYCAN STRUCTURES USING HIGH THROUGHPUT TECHNIQUES: GLYCAN MICROARRAY ANALYSIS USING RECOMBINANT PROTEIN AND WHOLE CELLSANUK INDRARATNA, DAVID DE OLIVEIRA, LAUREN E HARTLEY -TASSELL, CHRISTOPHER J DAY, MARK J WALKER, MICHAEL P JENNINGS, AND MARTINA L SANDERSON-SMITH PART III: ASSAYS TO INVESTIGATE GROUP A STREPTOCOCCUS VIRULENCE FACTORS AND VIRULENCE MECHANISMS11. USING LACTOCOCCUS LACTIS AS SURROGATE ORGANISM TO STUDY GROUP A STREPTOCOCCUS SURFACE PROTEINSCATHERINE (JIA-YUN) TSAI, JACELYN M.S. LOH, SAM BLANCHETT, AND THOMAS PROFT 12. EXPRESSION AND PURIFICATION OF COLLAGEN-LIKE PROTEINS OF GROUP A STREPTOCOCCUSSLAWOMIR LUKOMSKI AND DUDLEY H. MCNITT 13. DETECTION OF FIBRONECTIN-BINDING PROTEINS OF STREPTOCOCCUS PYOGENES USING LIGAND BLOT ANALYSISMASANOBU NAKATA AND SHIGETADA KAWABATA 14. MORPHOLOGY AND ULTRASTRUCTURE OF GROUP A STREPTOCOCCUS BIOFILMSTATYANA A. DANILOVA, T.A. SMIRNOVA, G.A. DANILINA, A.A. ADZHIEVA, N.V. SHEVLYAGINA, AND V.G. ZHUKHOVITSKY 15. DYNAMIC INTERACTIONS OF GROUP A STREPTOCOCCUS WITH HOST MACROPHAGES ANGELICA RIESTRA AND J. ANDRES VALDERRAMA 16. IDENTIFICATION OF GROUP A STREPTOCOCCUS-CONTAINING AUTOPHAGOSOME-LIKE VACUOLES TAKASHI NOZAWA AND ICHIRO NAKAGAWA 17. FLOW CYTOMETRY-BASED ASSAYS TO QUANTIFY COMPLEMENT DEPOSITION AND NEUTROPHIL UPTAKE OF GROUP A STREPTOCOCCUSNICOLA N.LYNSKEY 18. LIVE CELL MICROSCOPY AND FLOW CYTOMETRY TO STUDY STREPTOLYSIN S MEDIATED ERYTHROCYTE HEMOLYSIS DUSTIN L. HIGASHI, CHARLES R. TESSIER, AND SHAUN W. LEE 19. ISOLATION OF MONOCLONAL ANTIBODIES TO GROUP A STREPTOCOCCUS ANTIGENS USING PHAGE DISPLAYJEREMY M. RAYNES, MEI LIN TAY, SOK H. BY, JOHN D. STEEMSON, AND NICOLE J. MORELAND PART IV: GROUP A STREPTOCOCCUS KILLING ASSAYS AND INFECTION MODELS 20. ASSAYS TO ANALYSE ADHESION OF GROUP A STREPTOCOCCUS TO HOST CELLSADRINA H. J. KHEMLANI, THOMAS PROFT, AND JACELYN M.S. LOH 21. THE USE OF GALLERIA MELLONELLA (WAX MOTH) AS AN INFECTION MODEL FOR GROUP A STREPTOCOCCUSCATHERINE (JIA-YUN) TSAI, JACELYN M.S. LOH, AND THOMAS PROFT 22. A SUPERFICIAL SKIN SCARIFICATION METHOD IN MICE TO MIMIC STREPTOCOCCUS PYOGENES SKIN INFECTION IN HUMANSMANISHA PANDEY AND MICHAEL F GOOD 23. A MOUSE NASOPHARYNGEAL COLONISATION MODEL FOR GROUP A STREPTOCOCCUSADRINA H. J. KHEMLANI, THOMAS PROFT, AND JACELYN M. S. LOH 24. HUMANISED PLASMINOGEN MOUSE MODEL TO STUDY GROUP A STREPTOCOCCUS INVASIVE DISEASETANIA RIVERA-HERNANDEZ AND MARK J. WALKER 25. LACEFIELD WHOLE BLOOD KILLING ASSAY TO EVALUATE VACCINE EFFICACYMARK REGLINSKI26. AN OPSONOPHAGOCYTIC KILLING ASSAY FOR THE EVALUATION OF GROUP A STREPTOCOCCUS VACCINE ANTISERAREUBEN MCGREGOR , SCOTT JONES , JEREMY RAYNES , DAVID GOLDBLATT , AND NICOLE J. MORELAND PART V: GROUP A STREPTOCOCCUS THERAPIES27. VALIDATION OF SUITABLE CARRIER MOLECULES AND TARGET GENES FOR ANTISENSE THERAPY USING PEPTIDE-COUPLED PEPTIDE NUCLEIC ACIDS (PNAS) IN STREPTOCOCCIGINA BARKOWSKY, BERND KREIKEMEYER, AND NADJA PATENGE 28. INTERFERING WITH THE FOLDING OF GROUP A STREPTOCOCCAL PILI PROTEINSFERNANDA CONTRERAS AND JAIME ANDRES RIVAS-PARDO PART VI: APPLICATION OF GROUP A STREPTOCOCCUS-DERIVED METHODS29. USE OF STREPTOLYSIN O (SLO) TO STUDY THE FUNCTION OF LIPID RAFTSHEATHER E. MILLER 30. ENGINEERING OF GROUP A STREPTOCOCCUS ISOPEPTIDE BONDS INTO IMMUNOGLOBULIN-LIKE PROTEIN DOMAINSPAUL G. YOUNG AND EDWARD N. BAKER

THIS VOLUME DETAILS GROUP A STREPTOCOCCUS (GAS) RESEARCH AND PROVIDES THE READER WITH AN EXTENSIVE COLLECTION OF RESEARCH PROTOCOLS WITHIN THIS IMPORTANT FIELD. CHAPTERS GUIDE READERS THROUGH STANDARD GENETIC PROTOCOLS SUCH AS WHOLE GENOME SEQUENCING, TRANSCRIPTOME ANALYSES, PROTEOME ANALYSIS, APPLICATION OF GAS-DERIVED MOLECULAR TOOLS, AND METHODS THAT ARE CRUCIAL FOR THE EVALUATION OF NOVEL GAS VACCINES AND GAS VIRULENCE FACTORS, INCLUDING BACTERICIDAL ASSAYS AND ANIMAL INFECTION MODELS. WRITTEN IN THE HIGHLY SUCCESSFUL METHODS IN MOLECULAR BIOLOGY SERIES FORMAT, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS. AUTHORITATIVE AND CUTTING-EDGE, GROUP A STREPTOCOCCUS: METHODS AND PROTOCOLS AIMS TO ENSURE SUCCESSFUL RESULTS IN THE FURTHER STUDY OF THIS VITAL FIELD.

BIOLOGIA
MALALTIES INFECCIOSES
MICROBIOLOGIA
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